In a not so recent paper (January 2011), a nice report by Ann-Shyn Chiang group was published in PNAS.
Authors use both pharmacology and genetic trick to show that serotonin is involved in an aversive olfactory memory phase called ARM. Serotonin seems released by the DPM neuron (together with the amnesiac neuropeptide?), and the information seem to be received in the alpha/beta lobes of the mushroom bodies via the 5HT receptor A.
Three hours after training, it is known that the aversive memory retention score (after one cycle of conditioning) depends on two memory systems: a labile, cold shock sensitive memory, and ARM (anesthesia resistant memory, this latter one does not depend on de nuovo protein synthesis). Authors used cold shock to show that their treatments are affecting ARM and not the labile phase.
They localized the cell responsible for this effect using DDC-RNAi: it is the famous DPM neurons, known to have gap-junction with the GABAergic APL neuron (important for short term memory) and previously discribed as the neuron expressing amnesiac, a neuropeptide also involved in memory formation. They use three different driver line to block activity of or prevent serotonin production in these neurons, which both lead to a phenocopy of the serotonin block.
Finally, they used RNAi to show that the 5HT receptor A is necessary in alpha/beta lobes for the formation of ARM.
Lee, P., Lin, H., Chang, Y., Fu, T., Dubnau, J., Hirsh, J., Lee, T., & Chiang, A. (2011). Serotonin-mushroom body circuit modulating the formation of anesthesia-resistant memory in Drosophila Proceedings of the National Academy of Sciences, 108 (33), 13794-13799 DOI: 10.1073/pnas.1019483108
Tags: ARM, DPM neurons, serotonin
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Am 28. November 2011 um 20:58 Uhr
A nice study indeed, we try, but could not find any way to criticize the experiments or the interpretation.
I will therefore see to write a review for F1000.
Am 15. September 2022 um 06:12 Uhr
How big is the ARM memory?