Quick, de-interleave it! There are one-liners that never get old. This is one of them. $ perl -MBio::SeqIO -e ‘my $seqin = Bio::SeqIO-> new(-fh => \*STDIN, -format => ‘fasta’); while (my $seq = $seqin-> next_seq) { print “>”, $seq-> id, “\n”, $seq-> seq, “\n”; }’ < interleaved.fasta > deinterleaved.fasta Update 28-Jan-2019: Over time, I […]