Michael Grünstäudl (Gruenstaeudl), PhD

Researcher at the Freie Universität Berlin

Archiv der Kategorie 'one-liners'

Quick info parsing from GenBank accessions

Taking the essence. Have you ever found yourself browsing through individual sequence records of the NCBI GenBank database and wishing that you could extract only the metadata information of a record (e.g., authors, publication status, taxonomy), but not the feature table of a record or the sequence itself? With the help of Entrez Direct and […]

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One-liner: Interleaved to deinterleaved FASTA

Quick, de-interleave it! There are one-liners that never get old. This is one of them. $ perl -MBio::SeqIO -e ‚my $seqin = Bio::SeqIO-> new(-fh => \*STDIN, -format => ‚fasta‘); while (my $seq = $seqin-> next_seq) { print „>“, $seq-> id, „\n“, $seq-> seq, „\n“; }‘ < interleaved.fasta > deinterleaved.fasta

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Few-liner: Batch download of DNA sequences from NCBI

The wonders of entrez Today I found myself in need of a script to download dozens of DNA sequences submitted to NCBI Nucleotide. The sequences in questeion were stores in file input.txt. $ cat input.txt Liriope_muscari_USACult,JX080424 Dracaena_adamii_IVORYCOAST,JX080436 … Here is how I did it: $ INF=input.txt $ for line in $(cat $INF); do SEQNAME=$(echo „$line“ […]

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Bioinformatic spring cleaning – Part II

An improved few-liner to keep the data compressed If you wish to recusively loop through a folder and its nested subfolders and automatically gzip all files greater than 1 GB, the following few-liner is for you: for file in $(LANG=C find . -size +1G -type f -print); do if [[ ! $file == *.gzip ]]; […]

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Bioinformatic spring cleaning – Part I

A short one-liner to keep the data compressed One of the bash one-liners that I use after every successful project, yet never remember when needed, is for the simple task of looping through your folders, tar-zipping them and then removing the original folders. for i in $(ls -d */); do tar czf ${i%%/}.tar.gz $i && […]

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Alignment Phy2Nex few-liner

Alignment file format conversion for the efficient – Part II Today, I needed to convert a series of alignments, which were stored in the phylip format, into the common nexus format. The output DNA alignment hereby needed to be of sequential format (i.e., non-interleaved). In February 2017, I had already written a few-liner to conduct […]

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Trees Nex2Phy one-liner

Tree file format conversion for the efficient Today, I needed to convert a series of phylogenetic trees, which were stored in the common nexus format, into newick format. In order to do this efficiently, I wrote the following one-liner. Change into a directory containing your nexus-formatted tree files, then enter in your bash shell: R […]

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